Yazisa, Ukuxhobisa, Qhagamshela

Igama lam ndinguTyler Barr kwaye ndikwinyanga yokugqibela yePhD yam, kwiDyunivesithi yaseLeeds. Uphando lwam lwePhD luphantsi kweliso likaNjingalwazi Graham Cook kunye noGqr Fiona Errington-Mais. Umdla wam ku umhlaza Uphando lwe-immunotherapy lwaqala emva kokuba ndigqibe i-MSc kwi-Molecular Medicine. Ukuphulukana nomhlobo omangalisayo, u-Will, ku-Ewing sarcoma ngo-2015, xa kwapapashwa isifundi se-PhD siphanda ngonyango lwe-immunotherapies ye-Ewing sarcoma, kwakubonakala ngathi yenzelwe ukuba ibe njalo. Ndafaka isicelo ndaza ndaphumelela. Kule bhlog imfutshane, ndiyathemba ukucacisa kancinci malunga nophando lwam lwePhD, kunye nokuba oku kuya kwandiswa njani kube yi-osteosarcoma (OS).  

Uphando lwePhD  

Uphando lwam lwe-PhD luphande ukusetyenziswa kweentsholongwane ze-oncolytic (OV) kunyango lwe Ukuthunga isarcoma (uhlobo lomhlaza wamathambo). Iintsholongwane ze-Oncolytic 'zokubulala umhlaza' luhlobo lwe-immunotherapy. Banokubulala iiseli zomhlaza ngokuthe ngqo nangokuvula umzimba inkqubo yomzimba ukulwa nomhlaza.  

Sasisebenzisa ithumba iiseli ezikhule kwilebhu ukuvavanya ukuba zisebenza kangakanani na ii-OVs ekubulaleni iiseli ze-Ewing sarcoma ngokuthe ngqo. Siphinde sakhupha iiseli zomzimba (ezaziwa ngokuba ziiseli ezimhlophe zegazi) kwigazi eliqokelelwe ngokunikela ngegazi. Emva koko siye sajonga ukuba ii-OVs zinokuzisebenzisa njani ezi seli ze-immune kwaye zincede ukubulawa kweeseli zethumba. Olu phando lukwinqanaba lokuqala, kodwa la manyathelo ayimfuneko kulo naluphi na ukuqhubela phambili. Kwiminyaka ezayo, zinokuthi zisetyenziswe kulingo lwezonyango.  

Ukuphanda iintsholongwane ze-oncolytic kwi-osteosarcoma  

Uphando lwam lwasemva kobugqirha luya kuvavanya i-OV kwi-OS. Ii-OVs zijolise kwiiseli zomhlaza kwaye zishiya iiseli ezisempilweni zingonzakalanga ngenxa yezinto ezininzi ezahlukileyo phakathi kwezi ntlobo zeeseli. Enye yazo kubukho beemolekyuli ezincinci kumphezulu weseli yeeseli zomhlaza, ezivumela ukuba intsholongwane ingene kwiseli. Ukuchonga ii-OV ezinokusebenza kunyango lwe-OS, iiseli ezikhule elebhu ziya kujongwa kwezi molekyuli zincinci zokungena kwintsholongwane kumphezulu weseli. Ndiza kuvavanya ukubanakho kwe-OV ukubulala iiseli ze-OS ngokuthe ngqo. 

Ukuvavanya impembelelo yonyango lwe-OV kwiiseli zokhuselo lomzimba kwi-OS, ndiya kufumana iiseli zokuzikhusela kwigazi. Iiseli zokhuselo lomzimba ziya kuthi emva koko zinyangwe ngee-OVs kwaye zidibane neeseli ze-OS kwilebhu, ukufumanisa ukuba unyango lwe-OV luyakwandisa ukukwazi kwabo ukuqaphela kunye nokubulala iiseli zomhlaza.  

Iiseli zomhlaza ziyaziwa ukuba buthathaka impendulo yomzimba. Esi sesinye sezizathu eziphambili zokuba amathumba angakhula kwaye asasazeke. Uphando lwam luya kuphanda iindlela amathumba e-OS anokuzifihla ngayo kumajoni omzimba. Sakuba sikuqonde ngcono oku, kuyakusivumela ukuba sivavanye indibaniselwano ye-OV nolunye unyango ukuze sifumane olona lungcono.  

Impembelelo yexesha elide yolu phando  

Ilebhu yethu inethemba lokupapasha iziphumo zethu eziseka ukusebenza kwe-OV ngokuchasene ne-OS ukuze ezi zibe nokuqwalaselwa kulingo lwezonyango. Ukufumana unyango olutsha oluvunyiweyo ukuba lusetyenziswe yinkqubo ende kakhulu. Umsebenzi endiwenzayo linyathelo lokuqala elingundoqo ukuya koku. 

Ndihlala ndiyichaza njengeprojekthi nganye yophando ibonelela ngempendulo kwinxalenye yephazili entsonkothileyo. Iiprojekthi ezingakumbi zisebenza kwiindawo ezithile zophando lomhlaza, kokukhona sinokubeka ezo ziqwenga ngokukhawuleza. Ke ngoko, siya kuwuqonda ngcono umhlaza kwaye sichonge unyango olunobubele nolungcono kwizigulana.  

'Ndidibene nezigulane ezininzi ze-sarcoma kunye neentsapho zabo kule minyaka mi-4 idlulileyo. Akukho nto inika inkuthazo eyongezelelekileyo yokusebenza ekuphuhliseni unyango olungcono nolunobubele'. -UTyler Barr

Imbono yekamva  

Ndiyathemba ukuqhubeka nomsebenzi wophando, ngokugxila kwi-immunotherapies ye-sarcoma. Ndidibene nezigulane ezininzi ze-sarcoma kule minyaka mi-4 idlulileyo, ndizimisele kakhulu ukusebenzela ekufumaneni unyango olutsha. Kukho imfuno engxamisekileyo njengoko unyango olusemgangathweni lwangoku lwe-sarcomas luphelelwe lixesha. 

Ndidibene nabaphandi abaninzi abamangalisayo abavela kwihlabathi liphela. Ndinethemba elikhulu lokuba ngale ndlela yentsebenziswano, iminyaka ezayo ibambe isithembiso sokubonelela ngonyango oluphuculweyo lwe-OS kunye ne-Ewing sarcoma. Inkxaso yesisa ezifana Osteosarcoma ngoku ibalulekile ekuqhubeleni phambili uphando kulo mmandla.